quant it picogreen dsdna assay kit. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. quant it picogreen dsdna assay kit

 
 DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7quant it picogreen dsdna assay kit Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493

The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . The assay is performed at room temperature, and the signal is stable for 3 hours. Product Type. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Nucleic acid quantitation using Quant-iT Reagents. no. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Price from $9. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. Quant-iT™ PicoGreen ® dsDNA Assay Kit. 2. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Katalognummer: P11495. Description. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. Figure 1. (The same tips can be used. quant it picogreen dsdna kit - by Bioz Stars , 2023-09. no. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. No. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. 99 to $1999. Barcoded amplicons were pooled in. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. Revised: 10–June–2008 | MP 07581 Table 1. AccuBlue® RNA Quantitation Kit Details. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Picogreen assays are compatible with most microplate readers and fluorometers. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. 50 pg to 2 µg. Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. Picogreen assays are compatible with most microplate readers and fluorometers. 0 ml 200x in DMSO b. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. • Specific for dsDNA in the presence of RNA. Materials: 1. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. 95 mL TE for the . Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Price from $9. 3,201 3. 20 and 2. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The fluorescence intensity of PicoGreen was measured to determine the. 10 x 100 µL. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). This protocol is a simplified and condensed. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. '. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 3. ZERO BIAS - scores, article reviews, protocol conditions and morePicogreen assay with Qubit platform is considered to be the method of choice for DNA quantification. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Using 20x TE buffer make 1x solution. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Average 86 stars, based on 1. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. The PicoGreen is sticking to the sides of the cuvette or assay plate. The libraries were sequenced using the Illumina HiSeq 2500. Picogreen assays are compatible with most microplate readers and fluorometers. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. The normalized DNA was aliquoted into multiple tubes at 50µL volume. Rapidly detect as little as 0. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. Rapidly detect as little as 0. 995 mL TE for the . Three kits covering a wide range of dsDNA concentrations. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. PicoGreen dsDNA quantitation reagents are. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Tweet. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. DNA. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 86 / 100 stars. P7589. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. 15日开始对本试剂盒内标品(B组分)进行更新,由原来的小牛胸腺DNA升级为Lamda DNA,内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). Quantitation Range. Bioz Stars score: 86/100, based on 1 PubMed citations. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. 50 pg to 2 µg. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). 0. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. No. Quant-iT PicoGreen dsDNA Assay Kit. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Catalog Number. 5) prepared from 20x. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. 1 mL kit. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Picogreen assays are compatible with most microplate readers and fluorometers. The accuracy index is defined in Table 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 0 fluorometer according to the manufacturer’s protocols; a. • Suitable for use in 96- and 384-well plate formats. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. • Suitable for use in 96- and 384-well plate formats. 2. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. 10pg/µl vs. Figure 2. Add 5uL of PicoGreen reagent for every 1mL of buffer. 12. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. Briefly, 2 µL of sample was diluted in 498 µL TE. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. 99 to $1999. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. Qubit® dsDNA HS Assay Kit. quant it picogreen dsdna assay kit reagents. Rapidly detect as little as 0. PicoGreen定 量检测方法简单、方便,被多家生物制品厂所选择,成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光,无DNA不发荧光;所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出,PicoGreen定量DNA的方法检出限约0. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. UV흡광 결과보다 민감도 우수. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Quant-iTTM PicoGreen® Assay Reagent 0. 86100 stars. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Picogreen assays are compatible with most microplate readers and fluorometers. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. P7589. Picogreen assays are compatible with most microplate readers and fluorometers. 99 to $1999. 4). The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. This protocol is a simplified and c. 适用于(设备). Skip to main list 美谷分子仪器(上海)有限公司. Qubit DNA and RNA assays are available with different quantification ranges. • Suitable for use in 96- and 384-well plate formats. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に対応します。. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Description. Picogreen assays are compatible with most microplate readers and fluorometers. • Specific for dsDNA in the presence of RNA. Picogreen assays are compatible with most microplate readers and fluorometers. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 5. 激发/发射. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. 3. Optimized for fluorescence microplate readers. Catalog Number. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 1. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. 2. • Suitable for use in 96- and 384-well plate formats. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. PicoGreen dsDNA quantitation reagents are. 0 mL to 200 uL to fit a 96-well microplate format. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. • Specific for dsDNA in the presence of RNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Quant-iT RNA BR (Broad Range) Assay Kit. Table 3. 1. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. 3 Q33120 39. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. assay. 99. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Libraries were sequenced on the Illumina NovaSeq. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 10. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. • Suitable for use in 96- and 384-well plate formats. Rapidly detect as little as 0. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. Figure 2. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. 2. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. Rapidly detect as little as 0. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. Pico488 allows to quantitate as little as low pg/mL of dsDNA. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. 反应次数. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 9. Catalog Number. The assay is linear over three orders of magnitude and has little sequence. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. 2–100 ng of DNA. Highly selective for dsDNA over ssDNA or RNA. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. 作成者 LatB Staff 01. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Fluorescence was measured at. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. Quant-iT PicoGreen dsDNA Reagent. Prepare the assay. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). low-range. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. • Specific for dsDNA in the presence of RNA. P11495. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Bioz Stars score: 86/100, based on 1 PubMed citations. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. 文件. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Reagent. ru by: 1. DNA standard curve. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. High Sensitivity and Broad Range dsDNA Quantitation Superior sensitivity and broad dynamic range A ccuBlue™ dsDNA quantitation kits offer ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. • Suitable for use in 96- and 384-well plate formats. 04 corresponds to 2 μ g/mL dsDNA solution. Sample fluorescence was. Control samples were not lysed and consisted of free pDNA. 产品概述. For Research Use. According to the obtained results, the assay presents a linear correlation in a wide range of 0. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Prepare only the amount of 1:25 diluted PicoGreen required for that run. VIEWS. (2012). Briefly, 2 µL of sample was diluted in 498 µL TE buffer. 2 – 100 ng. 8 Measurement of growth factor content in. Unit Size. Picogreen assays are compatible with most microplate readers and fluorometers. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. dsDNA만을 선택적으로 정량. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. The Quant-iT PicoGreen dsDNA. PicoGreen assay. 9 Q33230 9 500 28. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. • Suitable for use in 96- and 384-well plate formats. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Serial dilutions were made to give appropriate cell, seeding numbers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Buy from Supplier. 製品概要. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. ZERO BIAS . The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. Picogreen assays are compatible with most microplate readers and fluorometers. io |. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. Description. Figure 8. 3ng/mL,DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Supplier: Invitrogen™ P11496. a)Use 110 sample number per plate. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Mix by gently vortexing for 5 seconds. 50 pg to 2 µg. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 99. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. No. • Suitable for use in 96- and 384-well plate formats. Unit Size. 5). Picogreen assays are compatible with most microplate readers and fluorometers. Product Type. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 1X dsDNA assay (%) 100 17. Quant It. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. In case dsDNA standards are available only the quantification reagent can be used. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Quant-iT PicoGreen dsDNA Assay Kit a. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification.